[Comment: Propiconazole is a fungicide that acts through demethylation. This study shows that -term exposure to higher concentrations of propiconazole affects the health status of fish. For more information on this chemical, please see http://www.pesticideinfo.org/Detail_Chemical.jsp?Rec_Id=PC34271]
Biochemical and physiological responses in liver and muscle of rainbow trout after long-term exposure to propiconazole
Zhi-Hua Lia, b, , , Vladimir Zlabeka, Ping Lia, b, Roman Grabica, c, Josef Veliseka, Jana Machovaa and Tomas Randaka. Ecotoxicology and Environmental Safety. Article in Press, Corrected Proof. doi:10.1016/j.ecoenv.2010.05.017.
a University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, 389 25 Vodnany, Czech Republic
b Key Field Station for Fishery Resource and Environment in Upper–Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000, China
c Department of Chemistry, Umea University, SE-90187 Umea, Sweden
Received 12 January 2010; revised 12 May 2010; accepted 18 May 2010. Available online 10 July 2010.
Abstract
In this study, the chronic toxic effects of PCZ, a triazole fungicide commonly present in surface and ground water, on morphological indices, ROS generation and RNA/DNA ratio in liver and white muscle of rainbow trout were investigated.
Zhi-Hua Lia, b, , , Vladimir Zlabeka, Ping Lia, b, Roman Grabica, c, Josef Veliseka, Jana Machovaa and Tomas Randaka. Ecotoxicology and Environmental Safety. Article in Press, Corrected Proof. doi:10.1016/j.ecoenv.2010.05.017.
a University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, 389 25 Vodnany, Czech Republic
b Key Field Station for Fishery Resource and Environment in Upper–Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000, China
c Department of Chemistry, Umea University, SE-90187 Umea, Sweden
Received 12 January 2010; revised 12 May 2010; accepted 18 May 2010. Available online 10 July 2010.
Abstract
In this study, the chronic toxic effects of PCZ, a triazole fungicide commonly present in surface and ground water, on morphological indices, ROS generation and RNA/DNA ratio in liver and white muscle of rainbow trout were investigated.
Fish were exposed at sublethal concentrations of PCZ (0.2, 50 and 500 μg L−1) for 7, 20 and 30 d. Compared with the control, there were significant lower CF and HSI in fish exposed at the highest concentration of PCZ.
ROS levels in both tissues increased significantly at higher PCZ concentrations (50 and 500 μg L−1) after 20 d and above, as well as in muscle of fish exposed at lowest PCZ concentration (0.2 μg L−1) after 30 d. The hepatic antioxidant enzymes (SOD and CAT) activities were induced significantly at higher concentrations (50, 500 μg L−1) of PCZ after 20 d and at 50 μg L−1 after 30 d. Additional, hepatic SOD activity was significantly induced at 0.2 μg L−1 after 30 d.
Compared with the hepatic antioxidant enzymes activities in fish exposed to 50 μg L−1 of PCZ, there was a decreasing trend in those exposed to 500 μg L−1 after 30 d exposure. However, both the antioxidant enzymes activities were significantly inhibited in muscle of fish exposed to 500 μg L−1 PCZ after 30 d. Moreover there was significant lower RNA/DNA ratio in both tissues after long-term exposure to higher concentration of PCZ.
In short, environmental concentrations of PCZ could not induce obvious impacts on fish, but long-term exposure to higher concentrations of CBZ could affect seriously the health status of fish.
