Neurotoxicity of fungal volatile organic compounds in Drosophila melanogaster.
Inamdar AA, Masurekar P, Bennett JW. Toxicol Sci. 2010 Jul 19. [Epub ahead of print]
Department of Plant Biology and Pathology, Rutgers University, New Jersey.
Abstract
Many volatile organic compounds (VOCs) are found in indoor environment as products of microbial metabolism. In damp indoor environments, fungi are associated with poor air quality. Some epidemiological studies have suggested that microbial VOCs have a negative impact on human health. Our study was designed to provide a reductionist approach towards studying fungal VOC-mediated toxicity using the inexpensive model organism, Drosophila melanogaster and pure chemical standards of several important fungal VOCs. Low concentrations of the following known fungal VOCs: 0.1% of 1-octen-3-ol and 0.5% of 2-octanone, 2,5 dimethylfuran, 3-octanol and trans-2-octenal caused locomotory defects and changes in GFP- and antigen-labeled dopaminergic neurons in adult Drosophila melanogaster. Locomotory defects could be partially rescued with L-DOPA. Ingestion of the antioxidant, Vitamin E, improved the survival span and delayed the VOC-mediated changes in dopaminergic neurons indicating that the VOC-mediated toxicity was due, in part, to generation of reactive oxygen species.
PMID: 20643751 [PubMed - as supplied by publisher]
