Cloudy apple juice protects against chemical-induced oxidative stress in rat.
Kujawska M, Ignatowicz E, Ewertowska M, Markowski J, Jodynis-Liebert J.
Kujawska M, Ignatowicz E, Ewertowska M, Markowski J, Jodynis-Liebert J.
Eur J Nutr. 2010 May 21. [Epub ahead of print]
Department of Toxicology, Poznań University of Medical Sciences, 30 Dojazd Str, 60-631, Poznań, Poland.
Abstract
BACKGROUND: Apples abundant in phenolic compounds show a variety of biological activities that may contribute to beneficial effects against some chronic diseases.
PURPOSE: The aim of our study was to assess the protective effect of cloudy apple juice against chemical-induced oxidative stress in rats.
METHODS: Male Wistar rats were treated with apple juice per os, 10 mL/kg/day for 28 days and with a single dose of N-nitrosodiethylamine (NDEA), 150 mg/kg or carbon tetrachloride (CCl(4)), 2 mL/kg, 24 h before killing. Two groups of rats not pretreated with juice were administered each of the xenobiotics alone.
RESULTS: Microsomal lipid peroxidation in the liver was decreased in rats pretreated with juice by 52-87% when compared to animals given NDEA or CCl(4) alone. Pretreatment with juice protected antioxidant enzymes: catalase, glutathione peroxidase and glutathione reductase but not superoxide dismutase. Their activity was recovered by 49-173% when compared to that in rats given either toxicant alone. The plasma activity of paraoxonase1 was reduced by both toxicants and was increased by 23% in the apple/CCl(4) group. A rise in plasma protein carbonyls caused by the xenobiotics was reduced by 20% only in apple/NDEA-treated rats. Also, in this group of animals, a 9% decrease in DNA damage in blood leukocytes was observed.
CONCLUSION: Phytochemicals in commonly consumed apple juice may protect some macromolecules against oxidative insult induced by xenobiotics.
PMID: 20490519 [PubMed - as supplied by publisher]
