Neurosci Lett. 2009 May 22;455(3):173-7.
Epub 2009 Mar 24
Continuous exposure to 900MHz GSM-modulated EMF alters morphological
maturation of neural cells.
http://www.ncbi.nlm.nih.gov/pubmed/19429115
Del Vecchio G, Giuliani A, Fernandez M, Mesirca P, Bersani F, Pinto R,
Ardoino L, Lovisolo GA, Giardino L, Calzà L.
BioPharmaNet-DIMORFIPA, Bologna University, Bologna, Italy.
The effects of radiofrequency electromagnetic field (RF-EMF) exposure
on neuronal phenotype maturation have been studied in two different in
vitro models: murine SN56 cholinergic cell line and rat primary
cortical neurons. The samples were exposed at a dose of 1W/kg at 900
MHz GSM modulated. The phenotype analysis was carried out at 48 and 72
h (24 and 48 h of SN56 cell line differentiation) or at 24, 72, 120 h
(2, 4 and 6 days in vitro for cortical neurons) of exposure, on live
and immunolabeled neurons, and included the morphological study of
neurite emission, outgrowth and branching. Moreover, cortical neurons
were studied to detect alterations in the expression pattern of
cytoskeleton regulating factors, e.g. beta-thymosin, and of early
genes, e.g. c-Fos and c-Jun through real-time PCR on mRNA extracted
after 24h exposure to EMF. We found that RF-EMF exposure reduced the
number of neurites generated by both cell systems, and this alteration
correlates to increased expression of beta-thymosin mRNA.
Epub 2009 Mar 24
Continuous exposure to 900MHz GSM-modulated EMF alters morphological
maturation of neural cells.
http://www.ncbi.nlm.nih.gov/pubmed/19429115
Del Vecchio G, Giuliani A, Fernandez M, Mesirca P, Bersani F, Pinto R,
Ardoino L, Lovisolo GA, Giardino L, Calzà L.
BioPharmaNet-DIMORFIPA, Bologna University, Bologna, Italy.
The effects of radiofrequency electromagnetic field (RF-EMF) exposure
on neuronal phenotype maturation have been studied in two different in
vitro models: murine SN56 cholinergic cell line and rat primary
cortical neurons. The samples were exposed at a dose of 1W/kg at 900
MHz GSM modulated. The phenotype analysis was carried out at 48 and 72
h (24 and 48 h of SN56 cell line differentiation) or at 24, 72, 120 h
(2, 4 and 6 days in vitro for cortical neurons) of exposure, on live
and immunolabeled neurons, and included the morphological study of
neurite emission, outgrowth and branching. Moreover, cortical neurons
were studied to detect alterations in the expression pattern of
cytoskeleton regulating factors, e.g. beta-thymosin, and of early
genes, e.g. c-Fos and c-Jun through real-time PCR on mRNA extracted
after 24h exposure to EMF. We found that RF-EMF exposure reduced the
number of neurites generated by both cell systems, and this alteration
correlates to increased expression of beta-thymosin mRNA.
